Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)



Document title: Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)
Journal: Brazilian archives of biology and technology
Database: PERIÓDICA
System number: 000392752
ISSN: 1516-8913
Authors: 1
1
1
2
Institutions: 1University of Tirana, Tirana. Albania
2University of Messina, Messina, Sicilia. Italia
Year:
Season: Sep-Oct
Volumen: 58
Number: 5
Pages: 750-756
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Analítico, descriptivo
English abstract Destabilisation of blood cell lysosomes in Mediterranean green crab Carcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 20 14 were exposed in the laboratory to sub - lethal, environmentally realistic concentrations of copper . Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p<0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p<0.05) than in control group, demonstrating the presence of stress on the animals. These results showed that C. aestuarii could be used as a successful and reliable bioindicator for evaluating the exposure to contaminants in laboratory conditions. NRRA provides a successful tool for rapid assessment of heavy metal pollution effects on marine biota
Disciplines: Biología
Keyword: Biología celular,
Crustáceos,
Ecología,
Carcinus aestuarii,
Contaminación,
Cobre,
Lisosomas,
Bioindicadores
Keyword: Biology,
Cell biology,
Crustaceans,
Ecology,
Carcinus aestuarii,
Pollution,
Copper,
Lysosomes,
Bioindicators
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