Revue: | Brazilian archives of biology and technology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000392752 |
ISSN: | 1516-8913 |
Autores: | Aliko, Valbona1 Hajdaraj, Gerta1 Caci, Atenisa1 Faggio, Caterina2 |
Instituciones: | 1University of Tirana, Tirana. Albania 2University of Messina, Messina, Sicilia. Italia |
Año: | 2015 |
Periodo: | Sep-Oct |
Volumen: | 58 |
Número: | 5 |
Paginación: | 750-756 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Analítico, descriptivo |
Resumen en inglés | Destabilisation of blood cell lysosomes in Mediterranean green crab Carcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 20 14 were exposed in the laboratory to sub - lethal, environmentally realistic concentrations of copper . Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p<0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p<0.05) than in control group, demonstrating the presence of stress on the animals. These results showed that C. aestuarii could be used as a successful and reliable bioindicator for evaluating the exposure to contaminants in laboratory conditions. NRRA provides a successful tool for rapid assessment of heavy metal pollution effects on marine biota |
Disciplinas: | Biología |
Palabras clave: | Biología celular, Crustáceos, Ecología, Carcinus aestuarii, Contaminación, Cobre, Lisosomas, Bioindicadores |
Keyword: | Biology, Cell biology, Crustaceans, Ecology, Carcinus aestuarii, Pollution, Copper, Lysosomes, Bioindicators |
Texte intégral: | Texto completo (Ver PDF) |