Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)



Título del documento: Copper Induced Lysosomal Membrane Destabilisation in Haemolymph Cells of Mediterranean Green Crab (Carcinus aestuarii, Nardo, 1847) from the Narta Lagoon (Albania)
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000392752
ISSN: 1516-8913
Autores: 1
1
1
2
Instituciones: 1University of Tirana, Tirana. Albania
2University of Messina, Messina, Sicilia. Italia
Año:
Periodo: Sep-Oct
Volumen: 58
Número: 5
Paginación: 750-756
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Analítico, descriptivo
Resumen en inglés Destabilisation of blood cell lysosomes in Mediterranean green crab Carcinus aestuarii was investigated using Neutral Red Retention Assay (NRRA). Crabs collected in Narta Lagoon, Vlora (Albania) during May 20 14 were exposed in the laboratory to sub - lethal, environmentally realistic concentrations of copper . Neutral Red Retention Time (NRRT) and glucose concentration in haemolymph of animals were measured. The mean NRRT showed a significant reduction for the animals of the treatment group compared to the control one (from 118.6 ± 28.4 to 36.4 ± 10.48 min, p<0.05), indicating damage of lysosomal membrane. Haemolymph glucose concentration was significantly higher in the treatment group (from 37.8 ± 2.7 to 137.8.4 ± 16.2 mg/dL, p<0.05) than in control group, demonstrating the presence of stress on the animals. These results showed that C. aestuarii could be used as a successful and reliable bioindicator for evaluating the exposure to contaminants in laboratory conditions. NRRA provides a successful tool for rapid assessment of heavy metal pollution effects on marine biota
Disciplinas: Biología
Palabras clave: Biología celular,
Crustáceos,
Ecología,
Carcinus aestuarii,
Contaminación,
Cobre,
Lisosomas,
Bioindicadores
Keyword: Biology,
Cell biology,
Crustaceans,
Ecology,
Carcinus aestuarii,
Pollution,
Copper,
Lysosomes,
Bioindicators
Texto completo: Texto completo (Ver PDF)