Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
Título del documento: Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000266084
ISSN: 0717-3458
Autores: 1
2
Instituciones: 1Shanghai Institute of Pharmaceutical Industry, Department of Bio-pharmaceutical, Shanghai. China
2Shanghai Academy of Agricultural Science, Department of Process and Store of Agro-products, Shanghai. China
Año:
Periodo: Abr
Volumen: 5
Número: 1
Paginación: 9-10
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts
Disciplinas: Medicina
Palabras clave: Farmacología,
Microbiología,
Biotecnología,
Agrobacterium tumefaciens,
Penicillium chrysogenum,
Vitreoscilla,
Genes,
Hemoglobina
Keyword: Medicine,
Microbiology,
Pharmacology,
Agrobacterium tumefaciens,
Penicillium chrysogenum,
Vitreoscilla,
Genes,
Hemoglobin
Texte intégral: Texto completo (Ver HTML)