Revista: | Electronic journal of biotechnology |
Base de datos: | PERIÓDICA |
Número de sistema: | 000266084 |
ISSN: | 0717-3458 |
Autores: | Chuan-Bao Sun1 Qiu-Lian Kong2 Wen-Si Xu |
Instituciones: | 1Shanghai Institute of Pharmaceutical Industry, Department of Bio-pharmaceutical, Shanghai. China 2Shanghai Academy of Agricultural Science, Department of Process and Store of Agro-products, Shanghai. China |
Año: | 2002 |
Periodo: | Abr |
Volumen: | 5 |
Número: | 1 |
Paginación: | 9-10 |
País: | Chile |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, analítico |
Resumen en inglés | The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts |
Disciplinas: | Medicina |
Palabras clave: | Farmacología, Microbiología, Biotecnología, Agrobacterium tumefaciens, Penicillium chrysogenum, Vitreoscilla, Genes, Hemoglobina |
Keyword: | Medicine, Microbiology, Pharmacology, Agrobacterium tumefaciens, Penicillium chrysogenum, Vitreoscilla, Genes, Hemoglobin |
Texto completo: | Texto completo (Ver HTML) |