Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
Document title: Efficient transformation of Penicillium chrysogenum mediated by Agrobacterium tumefaciens LBA4404 for cloning of Vitreoscilla hemoglobin gene
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000266084
ISSN: 0717-3458
Authors: 1
2
Institutions: 1Shanghai Institute of Pharmaceutical Industry, Department of Bio-pharmaceutical, Shanghai. China
2Shanghai Academy of Agricultural Science, Department of Process and Store of Agro-products, Shanghai. China
Year:
Season: Abr
Volumen: 5
Number: 1
Pages: 9-10
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract The vgb and bleomycin resistance genes could be efficiently transferred into the filamentous fungus Penicillium chrysogenum under help of T-DNA of Agrobacterium tumefaciens LBA4404 and the transferred genes were integrated at a chromosomal locus of P. chrysogenum. Transformation using A. tumefaciens LBA4404 could be enhanced in the presence of acetosyringone (AS) when being carried out in the conidiaand mycelium. The efficiencies of transformation could be improved up to 10 folds compared with conventional techniques for transformation of P. chrysogenum protoplasts
Disciplines: Medicina
Keyword: Farmacología,
Microbiología,
Biotecnología,
Agrobacterium tumefaciens,
Penicillium chrysogenum,
Vitreoscilla,
Genes,
Hemoglobina
Keyword: Medicine,
Microbiology,
Pharmacology,
Agrobacterium tumefaciens,
Penicillium chrysogenum,
Vitreoscilla,
Genes,
Hemoglobin
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