Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes
Document title: Intraspecific variation in the venom electrophoretic profile of recently captured Crotalus durissus terrificus (Laurenti, 1768) snakes
Journal: The journal of venomous animals and toxins
Database: PERIÓDICA
System number: 000298735
ISSN: 0104-7930
Authors: 1

2

3
Institutions: 1Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Botucatu, Sao Paulo. Brasil
2Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Botucatu, Sao Paulo. Brasil
3Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil
Year:
Season: Dic
Volumen: 7
Number: 2
Pages: 276-301
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females
Disciplines: Biología
Keyword: Reptiles,
Bioquímica,
Veneno de víbora,
Proteínas,
Electroforesis,
Variación intraespecífica,
Crotalus durissus terrificus,
Crotalidae
Keyword: Biology,
Reptiles,
Biochemistry,
Snake venom,
Protein composition,
Electrophoresis,
Intraspecific variation,
Crotalus durissus terrificus,
Crotalidae
Full text: Texto completo (Ver HTML)