| Revista: | The journal of venomous animals and toxins |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000298735 |
| ISSN: | 0104-7930 |
| Autores: | Magro, A.J1 Silva, R.J. da Ramos, P.R.R2 Cherubini, A.L Hatayde, M.R3 |
| Instituciones: | 1Universidade Estadual Paulista "Julio de Mesquita Filho", Centro de Estudos de Venenos e Animais Peconhentos, Botucatu, Sao Paulo. Brasil 2Universidade Estadual Paulista "Julio de Mesquita Filho", Instituto de Biociencias, Botucatu, Sao Paulo. Brasil 3Universidade Estadual Paulista "Julio de Mesquita Filho", Faculdade de Ciencias Agrarias e Veterinarias, Jaboticabal, Sao Paulo. Brasil |
| Año: | 2001 |
| Periodo: | Dic |
| Volumen: | 7 |
| Número: | 2 |
| Paginación: | 276-301 |
| País: | Brasil |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | The aim of this study was to compare individual venom samples of Crotalus durissus terrificus recently captured in the wild to evaluate possible differences in venom protein composition. Protein levels were quantified by biochemical method (Biuret) and then submitted to electrophoresis. Electrophoresis studies of native protein were performed in vertical slabs of polyacrylamide gel (PAGE), in an alkaline discontinuous buffer system, with a concentration of 10% in the separation gel. SDS-PAGE was performed in PhastGel® (8-25). Both gels were stained with Coomassie Blue. Gels were analyzed using the VDS-Pharmacia® device. Our results indicate that all analyzed venom samples showed different protein composition, although common protein fractions were detected in some individual samples. Differences were observed between the different individual venom samples and so in the same specimen in relation to the time of collection, for both techniques used. Diet did not influence the variability of venom composition. There is a significant difference in native venom protein composition of males and females |
| Disciplinas: | Biología |
| Palabras clave: | Reptiles, Bioquímica, Veneno de víbora, Proteínas, Electroforesis, Variación intraespecífica, Crotalus durissus terrificus, Crotalidae |
| Keyword: | Biology, Reptiles, Biochemistry, Snake venom, Protein composition, Electrophoresis, Intraspecific variation, Crotalus durissus terrificus, Crotalidae |
| Texto completo: | Texto completo (Ver HTML) |
