| Journal: | The journal of venomous animals and toxins |
| Database: | PERIÓDICA |
| System number: | 000298760 |
| ISSN: | 0104-7930 |
| Authors: | Lipps, B.V1 |
| Institutions: | 1Ophidia Products Inc., Houston, Texas. Estados Unidos de América |
| Year: | 2002 |
| Volumen: | 8 |
| Number: | 2 |
| Pages: | 214-255 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently, mouse bioassay is in use for toxin detection and assay. Binding affinity of IgG from anti-LT-10 showed a linear relationship with mouse bioassay by ELISA detection limit to some toxins only. This may be due to the fact that anti-LTNF and anti-LT-10 detected the toxins that were not lethal to mouse. Thus, anti-LTNF and anti-LT-10 can be useful in assaying toxins as an alternative to mouse bioassay |
| Disciplines: | Química, Biología |
| Keyword: | Inmunología, Toxinas, Veneno, Anticuerpos, ELISA, Factor neutralizante de toxinas letales |
| Keyword: | Chemistry, Biology, Immunology, Toxins, Antibodies, ELISA, Lethal toxin neutralizing factor, Venom |
| Full text: | Texto completo (Ver HTML) |
