In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor



Título del documento: In vitro assay of biological and chemical toxins using antibodies against lethal toxin neutralizing factor
Revista: The journal of venomous animals and toxins
Base de datos: PERIÓDICA
Número de sistema: 000298760
ISSN: 0104-7930
Autores: 1
Instituciones: 1Ophidia Products Inc., Houston, Texas. Estados Unidos de América
Año:
Volumen: 8
Número: 2
Paginación: 214-255
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Lethal Toxin Neutralizing Factor (N-LTNF), MW 63.0 kDa, was isolated from opossum serum. After trypsin digestion, the active domain of N-LTNF was isolated and sequenced. The synthetic peptide consisting of ten amino acids was designated as LT-10. N-LTNF and LT-10 inhibited the lethality of animal, plant and bacteria toxins when tested on mice non-immunologically. The antibodies against N-LTNF and LT-10 only reacted immunologically with toxins and not with non-toxic substances. Anti-LTNF and anti-LT-10 reacted immunologically by ELISA test with toxins that were not detected by mouse test, such as cholera toxin and digoxin. Anti-LTNF and anti-LT-10 failed to react immunologically with non-toxic substances, such as nerve growth factor and collagen. Currently, mouse bioassay is in use for toxin detection and assay. Binding affinity of IgG from anti-LT-10 showed a linear relationship with mouse bioassay by ELISA detection limit to some toxins only. This may be due to the fact that anti-LTNF and anti-LT-10 detected the toxins that were not lethal to mouse. Thus, anti-LTNF and anti-LT-10 can be useful in assaying toxins as an alternative to mouse bioassay
Disciplinas: Química,
Biología
Palabras clave: Inmunología,
Toxinas,
Veneno,
Anticuerpos,
ELISA,
Factor neutralizante de toxinas letales
Keyword: Chemistry,
Biology,
Immunology,
Toxins,
Antibodies,
ELISA,
Lethal toxin neutralizing factor,
Venom
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