| Journal: | The brazilian journal of infectious diseases |
| Database: | PERIÓDICA |
| System number: | 000290903 |
| ISSN: | 1413-8670 |
| Authors: | Guerreiro, Jaqueline B1 Porto, Maria Aurelia F Santos, Silvane B Lacerda, Lino Ho, John L2 Carvalho, Edgar Marcelino de3 |
| Institutions: | 1Universidade Federal da Bahia, Salvador, Bahia. Brasil 2Cornell University, Nueva York. Estados Unidos de América 3Universidade Federal da Bahia, Hospital Universitario Prof. Edgard Santos, Salvador, Bahia. Brasil |
| Year: | 2005 |
| Season: | Dic |
| Volumen: | 9 |
| Number: | 6 |
| Pages: | 510-514 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-g and TNF-a levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-g and TNF-a by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-g and TNF-a production, were significantly higher (p<0.001) in HTLV-1 infected subjects than in healthy individuals. A trend towards a positive correlation was noted, with increasing percentage of NBT positive neutrophils and levels of IFN-g. The high IFN-g producing HTLV-1 patient group had significantly greater NBT than healthy controls, 43±24% and 17±4.8% respectively (p< 0.001), while no significant difference was observed between healthy controls and the low IFN-g-producing HTLV-1 patient group (30±20%). Spontaneous neutrophil activation is another marker of immune perturbation resulting from HTLV-1 infection. In vivo activation of neutrophils observed in HTLV-1 infected subjects is likely to be the same process that causes spontaneous IFN-g production, or it may partially result from direct IFN-g stimulation |
| Disciplines: | Biología, Medicina |
| Keyword: | Inmunología, Virus, Hematología, Células T, Neutrófilos, Leucemia |
| Keyword: | Biology, Medicine, Immunology, Virus, Hematology, T cells, Neutrophils, Leukemia |
| Full text: | Texto completo (Ver HTML) |
