Spontaneous neutrophil activation in HTLV-1 infected patients



Título del documento: Spontaneous neutrophil activation in HTLV-1 infected patients
Revista: The brazilian journal of infectious diseases
Base de datos: PERIÓDICA
Número de sistema: 000290903
ISSN: 1413-8670
Autores: 1



2
3
Instituciones: 1Universidade Federal da Bahia, Salvador, Bahia. Brasil
2Cornell University, Nueva York. Estados Unidos de América
3Universidade Federal da Bahia, Hospital Universitario Prof. Edgard Santos, Salvador, Bahia. Brasil
Año:
Periodo: Dic
Volumen: 9
Número: 6
Paginación: 510-514
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Human T cell lymphotropic Virus type-1 (HTLV-1) induces lymphocyte activation and proliferation, but little is known about the innate immune response due to HTLV-1 infection. We evaluated the percentage of neutrophils that metabolize Nitroblue tetrazolium (NBT) to formazan in HTLV-1 infected subjects and the association between neutrophil activation and IFN-g and TNF-a levels. Blood was collected from 35 HTLV-1 carriers, from 8 patients with HAM/TSP (HTLV-1- associated myelopathy); 22 healthy individuals were evaluated for spontaneous and lipopolysaccharide (LPS)-stimulated neutrophil activity (reduction of NBT to formazan). The production of IFN-g and TNF-a by unstimulated mononuclear cells was determined by ELISA. Spontaneous NBT levels, as well as spontaneous IFN-g and TNF-a production, were significantly higher (p<0.001) in HTLV-1 infected subjects than in healthy individuals. A trend towards a positive correlation was noted, with increasing percentage of NBT positive neutrophils and levels of IFN-g. The high IFN-g producing HTLV-1 patient group had significantly greater NBT than healthy controls, 43±24% and 17±4.8% respectively (p< 0.001), while no significant difference was observed between healthy controls and the low IFN-g-producing HTLV-1 patient group (30±20%). Spontaneous neutrophil activation is another marker of immune perturbation resulting from HTLV-1 infection. In vivo activation of neutrophils observed in HTLV-1 infected subjects is likely to be the same process that causes spontaneous IFN-g production, or it may partially result from direct IFN-g stimulation
Disciplinas: Biología,
Medicina
Palabras clave: Inmunología,
Virus,
Hematología,
Células T,
Neutrófilos,
Leucemia
Keyword: Biology,
Medicine,
Immunology,
Virus,
Hematology,
T cells,
Neutrophils,
Leukemia
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