| Journal: | Revista mexicana de ciencias farmacéuticas |
| Database: | PERIÓDICA |
| System number: | 000384957 |
| ISSN: | 1870-0195 |
| Authors: | Lindley, David J1 Carl, Stephen M1 Mowery, Stephanie A1 Knipp, Gregory T1 |
| Institutions: | 1Purdue University, College of Pharmacy, West Lafayette, Indiana. Estados Unidos de América |
| Year: | 2011 |
| Season: | Oct-Dic |
| Volumen: | 42 |
| Number: | 4 |
| Pages: | 57-65 |
| Country: | México |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | There have been relatively few studies focused on the proton-dependent oligopeptide transporter (POT) superfamily member, Peptide/Histidine Transporter 1 (PHT1), with respect to its contribution to the ADME of peptides and peptide-based drugs. These studies were conducted to determine hPHT1-mediated, H+-dependent uptake kinetics of histidine, carnosine, Gly-Sar and valacyclovir in stably transfected hPHT1-COS-7 cells comparative to kinetics determined in an empty vector (Mock) stably transfected cell line. The results suggest that Gly-Sar appears to be a substrate for PHT1 based on efflux from the stably transfected hPHT1 COS-7 cells. Histidine and Gly-Sar concentration- and time-dependent studies suggest mixed-uptake kinetics. These studies suggest that stably transfected hPHT1-COS-7 cells exhibit different uptake kinetics than those observed in our previous studies and illustrate the requirement for experiments to delineate the physiological role of hPHT1 |
| Disciplines: | Medicina, Biología |
| Keyword: | Farmacología, Biología celular, Péptidos, Histidina, Transportadores, Absorción molecular |
| Keyword: | Medicine, Biology, Pharmacology, Cell biology, Peptides, Histidine, Transporters, Molecular absorption |
| Full text: | Texto completo (Ver HTML) |
