The evaluation of peptide/histidine transporter 1 (PHT1) function: uptake kinetics utilizing a cos-7 stably transfected cell line
Título del documento: The evaluation of peptide/histidine transporter 1 (PHT1) function: uptake kinetics utilizing a cos-7 stably transfected cell line
Revista: Revista mexicana de ciencias farmacéuticas
Base de datos: PERIÓDICA
Número de sistema: 000384957
ISSN: 1870-0195
Autores: 1
1
1
1
Instituciones: 1Purdue University, College of Pharmacy, West Lafayette, Indiana. Estados Unidos de América
Año:
Periodo: Oct-Dic
Volumen: 42
Número: 4
Paginación: 57-65
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés There have been relatively few studies focused on the proton-dependent oligopeptide transporter (POT) superfamily member, Peptide/Histidine Transporter 1 (PHT1), with respect to its contribution to the ADME of peptides and peptide-based drugs. These studies were conducted to determine hPHT1-mediated, H+-dependent uptake kinetics of histidine, carnosine, Gly-Sar and valacyclovir in stably transfected hPHT1-COS-7 cells comparative to kinetics determined in an empty vector (Mock) stably transfected cell line. The results suggest that Gly-Sar appears to be a substrate for PHT1 based on efflux from the stably transfected hPHT1 COS-7 cells. Histidine and Gly-Sar concentration- and time-dependent studies suggest mixed-uptake kinetics. These studies suggest that stably transfected hPHT1-COS-7 cells exhibit different uptake kinetics than those observed in our previous studies and illustrate the requirement for experiments to delineate the physiological role of hPHT1
Disciplinas: Medicina,
Biología
Palabras clave: Farmacología,
Biología celular,
Péptidos,
Histidina,
Transportadores,
Absorción molecular
Keyword: Medicine,
Biology,
Pharmacology,
Cell biology,
Peptides,
Histidine,
Transporters,
Molecular absorption
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