Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction
Document title: Evaluation of eleven immunochromatographic assays for SARS-CoV-2 detection: investigating the dengue cross-reaction
Journal: Revista do Instituto de Medicina Tropical de Sao Paulo
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System number: 000547881
ISSN: 0036-4665
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Institutions: 1Universidade de São Paulo, Faculdade de Medicina, São Paulo, São Paulo. Brasil
2Fundação Oswaldo Cruz, Instituto Carlos Chagas, Curitiba. Brasil
3Hospital Sírio Libanês, Unidade de Terapia Intensiva, São Paulo, São Paulo. Brasil
Year:
Volumen: 64
Country: Brasil
Language: Inglés
English abstract COVID-19 disease is spread worldwide and diagnostic techniques have been studied in order to contain the pandemic. Immunochromatographic (IC) assays are feasible and a low-cost alternative especially in low and middle-income countries, which lack structure to perform certain diagnostic techniques. Here we evaluate the sensitivity and specificity of eleven different IC tests in 145 serum samples from confirmed cases of COVID-19 using RT-PCR and 100 negative serum samples from blood donors collected in February 2019. We also evaluated the cross-reactivity with dengue using 20 serum samples from patients with confirmed diagnosis for dengue collected in early 2019 through four different tests. We found high sensitivity (92%), specificity (100%) and an almost perfect agreement (Kappa 0.92) of IC assay, especially when we evaluated IgG and IgM combined after 10 days from the onset of symptoms with RT-PCR. However, we detected cross-reactivity between dengue and COVID-19 mainly with IgM antibodies (5 to 20% of cross-reaction) and demonstrated the need for better studies about diagnostic techniques for these diseases.
Keyword: IC assays,
COVID-19,
Dengue,
Serology
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