| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000459442 |
| ISSN: | 0074-0276 |
| Authors: | Moreira, Leyllane Rafael1 Silva, Ana Carla2 Costa Oliveira, Cíntia Nascimento da2 Silva-Junior, Claudeir Dias da1 Nascimento, Amanda Vasconcelos2 Oliveira, Kamila Kássia dos Santos2 Soares, Ana Karine de Araújo3 Saraiva, Karina Lidianne Alcântara2 Paiva Cavalcanti, Milena de2 Lorena, Virginia Maria Barros de2 |
| Institutions: | 1Universidade Federal de Pernambuco, Programa de Pos-Graduacao em Medicina Tropical, Recife, Pernambuco. Brasil 2Fundacao Oswaldo Cruz, Instituto Aggeu Magalhaes, Recife, Pernambuco. Brasil 3Fundacao Altino Ventura, Recife, Pernambuco. Brasil |
| Year: | 2023 |
| Volumen: | 118 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | Trypanosoma cruzi, which causes Chagas disease (CD), is a versatile haemoparasite that uses several strategies to evade the host’s immune response, including adipose tissue (AT), used as a reservoir of infection. As it is an effective barrier to parasite evasion, the effectiveness of the drug recommended for treating CD, Benznidazole (BZ), may be questionable. OBJECTIVE To this end, we evaluated the parasite load and immunomodulation caused by BZ treatment in the culture of adipocytes differentiated from human adipose tissue-derived stem cells (ADSC) infected with T. cruzi. METHODS The ADSC were subjected to adipogenic differentiation. We then carried out four cultures in which we infected the differentiated AT with trypomastigote forms of the Y strain of T. cruzi and treated them with BZ. After the incubation, the infected AT was subjected to quantitative polymerase chain reaction (qPCR) to quantify the parasite load and transmission electron microscopy (TEM) to verify the infection. The supernatant was collected to measure cytokines, chemokines, and adipokines. FINDINGS We found elevated secretion of IL-6, CXCL-10/IP-10, CCL2/MCP-1, CCL5/RANTES, and leptin in infected fat cells. However, treatment with BZ promoted a decrease in IL-6. MAIN CONCLUSION Therefore, we believe that BZ has a beneficial role as it reduces inflammation in infected fat cells |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Inmunología, Farmacología, Benzonidazol, Inmunomodulación, Tejido adiposo, Trypanosoma cruzi |
| Keyword: | Parasitology, Immunology, Pharmacology, Adipose tissue, Trypanosoma cruzi, Immunomodulation, Benznidazole |
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