Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells



Título del documento: Benznidazole treatment decreases IL-6 levels in Trypanosoma cruzi-infected human adipocytes differentiated from adipose tissue-derived stem cells
Revista: Memorias do Instituto Oswaldo Cruz
Base de datos: PERIÓDICA
Número de sistema: 000459442
ISSN: 0074-0276
Autores: 1
2
2
1
2
2
3
2
2
2
Instituciones: 1Universidade Federal de Pernambuco, Programa de Pos-Graduacao em Medicina Tropical, Recife, Pernambuco. Brasil
2Fundacao Oswaldo Cruz, Instituto Aggeu Magalhaes, Recife, Pernambuco. Brasil
3Fundacao Altino Ventura, Recife, Pernambuco. Brasil
Año:
Volumen: 118
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Trypanosoma cruzi, which causes Chagas disease (CD), is a versatile haemoparasite that uses several strategies to evade the host’s immune response, including adipose tissue (AT), used as a reservoir of infection. As it is an effective barrier to parasite evasion, the effectiveness of the drug recommended for treating CD, Benznidazole (BZ), may be questionable. OBJECTIVE To this end, we evaluated the parasite load and immunomodulation caused by BZ treatment in the culture of adipocytes differentiated from human adipose tissue-derived stem cells (ADSC) infected with T. cruzi. METHODS The ADSC were subjected to adipogenic differentiation. We then carried out four cultures in which we infected the differentiated AT with trypomastigote forms of the Y strain of T. cruzi and treated them with BZ. After the incubation, the infected AT was subjected to quantitative polymerase chain reaction (qPCR) to quantify the parasite load and transmission electron microscopy (TEM) to verify the infection. The supernatant was collected to measure cytokines, chemokines, and adipokines. FINDINGS We found elevated secretion of IL-6, CXCL-10/IP-10, CCL2/MCP-1, CCL5/RANTES, and leptin in infected fat cells. However, treatment with BZ promoted a decrease in IL-6. MAIN CONCLUSION Therefore, we believe that BZ has a beneficial role as it reduces inflammation in infected fat cells
Disciplinas: Medicina
Palabras clave: Parasitología,
Inmunología,
Farmacología,
Benzonidazol,
Inmunomodulación,
Tejido adiposo,
Trypanosoma cruzi
Keyword: Parasitology,
Immunology,
Pharmacology,
Adipose tissue,
Trypanosoma cruzi,
Immunomodulation,
Benznidazole
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