| Journal: | Memorias do Instituto Oswaldo Cruz |
| Database: | PERIÓDICA |
| System number: | 000446391 |
| ISSN: | 0074-0276 |
| Authors: | Schappo, Ana Paula1 Bittencourt, Najara C2 Bertolla, Leticia P1 Forcellini, Sofia1 Silva, Ana Beatriz Iung Enembreck da1 Santos, Hellen Geremias dos1 Gervásio, João Henrique1 Lacerda, Marcus V.G3 Lopes, Stefanie C.P3 Costa, Fabio T.M2 Albrecht, Letusa1 |
| Institutions: | 1Fundacao Oswaldo Cruz, Instituto Carlos Chagas, Curitiba, Parana. Brasil 2Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil 3Fundacao Oswaldo Cruz, Instituto Leonidas e Maria Deane, Manaus, Amazonas. Brasil 4Fundacao de Medicina Tropical Dr. Heitor Vieira Dourado, Gerencia de Malaria, Manaus, Amazonas. Brasil |
| Year: | 2021 |
| Volumen: | 116 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | BACKGROUND Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions. OBJECTIVE To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E. METHODS Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells. FINDINGS Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1. MAIN CONCLUSIONS PvBrVIR-E was antigenic and adhesive to CHO-ICAM1 |
| Disciplines: | Medicina |
| Keyword: | Parasitología, Inmunología, Familia multigénica, Antigenicidad, Adhesión celular, Plasmodium vivax |
| Keyword: | Parasitology, Immunology, Multigene family, Antigenicity, Cell adhesion, Plasmodium vivax |
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