The effect of smok derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (orchidaceae)



Document title: The effect of smok derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (orchidaceae)
Journal: Lankesteriana
Database: PERIÓDICA
System number: 000424819
ISSN: 1409-3871
Authors: 1
1
1
Institutions: 1RMIT University, School of Applied Sciences, Bundoora, Victoria. Australia
Year:
Season: Dic
Volumen: 18
Number: 3
Pages: 167-175
Country: Costa Rica
Language: Inglés
Document type: Artículo
Approach: Experimental
English abstract Abstract . Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its decline in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and its mycorrhizal fungi. Stored seeds were germinated in vitro with peloton- isolated fungal isolates with varying concentrations of smoke water ( 0–1.0 mL L -1 ) to simulate fire and sucrose as the carbon source (10 g L -1 ). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges
Disciplines: Biología,
Agrociencias
Keyword: Botánica,
Fitotecnia,
Fisiología vegetal,
Cultivo in vitro,
Micorrizas,
Agua de humo,
Germinación simbiótica
Keyword: Botany,
Crop husbandry,
Plant physiology,
In vitro culture,
Mycorrhizae,
Smoke water,
Symbiotic germination
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