The effect of smok derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (orchidaceae)



Título del documento: The effect of smok derivatives and carbon utilisation on symbiotic germination of the endangered Pterostylis despectans (orchidaceae)
Revista: Lankesteriana
Base de datos: PERIÓDICA
Número de sistema: 000424819
ISSN: 1409-3871
Autores: 1
1
1
Instituciones: 1RMIT University, School of Applied Sciences, Bundoora, Victoria. Australia
Año:
Periodo: Dic
Volumen: 18
Número: 3
Paginación: 167-175
País: Costa Rica
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés Abstract . Orchids are highly dependent on exogenous nutritional sources and mycorrhizal associations to survive, particularly when challenged by extreme environmental stress such as bushfires that contribute significantly to its decline in nature. In this study, the effect of smoke derivatives and carbon utilisation was explored to improve germination and seedling establishment of an Australian endangered orchid, Pterostylis despectans (Lowly Greenhood) and its mycorrhizal fungi. Stored seeds were germinated in vitro with peloton- isolated fungal isolates with varying concentrations of smoke water ( 0–1.0 mL L -1 ) to simulate fire and sucrose as the carbon source (10 g L -1 ). Smoke water significantly increased germination, with advanced protocorms and robust seedlings produced. Sucrose inhibited germination such that protocorms and leafing was absent with sucrose inclusion. Fungal isolates were highly variable on its germination efficacy and tolerance to smoke water, highlighting the importance of fungal diversity and supports research-based conservation strategies to circumvent environmental challenges
Disciplinas: Biología,
Agrociencias
Palabras clave: Botánica,
Fitotecnia,
Fisiología vegetal,
Cultivo in vitro,
Micorrizas,
Agua de humo,
Germinación simbiótica
Keyword: Botany,
Crop husbandry,
Plant physiology,
In vitro culture,
Mycorrhizae,
Smoke water,
Symbiotic germination
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