| Journal: | Genetics and molecular biology |
| Database: | PERIÓDICA |
| System number: | 000459236 |
| ISSN: | 1415-4757 |
| Authors: | González Sayer, Sandra1 Oggenfuss, Ursula3 García, Ibonne2 Aristizabal, Fabio2 Croll, Daniel3 Riaño-Pachon, Diego M1 |
| Institutions: | 1Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, Sao Paulo. Brasil 2Universidad Nacional de Colombia, Instituto de Biotecnología, Bogotá. Colombia 3Neuchatel University, Laboratory of Evolutionary Genetics, Neuchatel. Suiza |
| Year: | 2022 |
| Volumen: | 45 |
| Number: | 1 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | Pseudocercospora ulei is the causal agent of South American Leaf Blight (SALB), the main disease affecting Hevea brasiliensis rubber tree, a native species to the Amazon. Rubber tree is a major crop in South American countries and SALB disease control strategies would benefit from the availability of genomic resources for the fungal pathogen. Here, we assembled and annotated the P. ulei genome. Shotgun sequencing was performed using second and third generation sequencing technologies. We present the first P. ulei high-quality genome assembly, the largest among Mycosphaerellaceae, with 93.8 Mbp, comprising 215 scaffolds, an N50 of 2.8 Mbp and a BUSCO gene completeness of 97.5%. We identified 12,745 protein-coding gene models in the P. ulei genome with 756 genes encoding secreted proteins and 113 genes encoding effector candidates. Most of the genome (80%) is composed of repetitive elements dominated by retrotransposons of the Gypsy superfamily. P. ulei has the largest genome size among Mycosphaerellaceae, with the highest TE content. In conclusion, we have established essential genomic resources for a wide range of studies on P. ulei and related species |
| Disciplines: | Biología |
| Keyword: | Genética, Hongos, Fitopatología, Hongos, Pseudocercospora ulei, Hevea brasiliensis, Caucho natural, Genoma |
| Keyword: | Genetics, Phytopathology, Fungi, Pseudocercospora ulei, Natural rubber, Hevea brasiliensis, Genome |
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