High-quality genome assembly of Pseudocercospora ulei the main threat to natural rubber trees



Título del documento: High-quality genome assembly of Pseudocercospora ulei the main threat to natural rubber trees
Revue: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459236
ISSN: 1415-4757
Autores: 1
3
2
2
3
1
Instituciones: 1Universidade de Sao Paulo, Centro de Energia Nuclear na Agricultura, Piracicaba, Sao Paulo. Brasil
2Universidad Nacional de Colombia, Instituto de Biotecnología, Bogotá. Colombia
3Neuchatel University, Laboratory of Evolutionary Genetics, Neuchatel. Suiza
Año:
Volumen: 45
Número: 1
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés Pseudocercospora ulei is the causal agent of South American Leaf Blight (SALB), the main disease affecting Hevea brasiliensis rubber tree, a native species to the Amazon. Rubber tree is a major crop in South American countries and SALB disease control strategies would benefit from the availability of genomic resources for the fungal pathogen. Here, we assembled and annotated the P. ulei genome. Shotgun sequencing was performed using second and third generation sequencing technologies. We present the first P. ulei high-quality genome assembly, the largest among Mycosphaerellaceae, with 93.8 Mbp, comprising 215 scaffolds, an N50 of 2.8 Mbp and a BUSCO gene completeness of 97.5%. We identified 12,745 protein-coding gene models in the P. ulei genome with 756 genes encoding secreted proteins and 113 genes encoding effector candidates. Most of the genome (80%) is composed of repetitive elements dominated by retrotransposons of the Gypsy superfamily. P. ulei has the largest genome size among Mycosphaerellaceae, with the highest TE content. In conclusion, we have established essential genomic resources for a wide range of studies on P. ulei and related species
Disciplinas: Biología
Palabras clave: Genética,
Hongos,
Fitopatología,
Hongos,
Pseudocercospora ulei,
Hevea brasiliensis,
Caucho natural,
Genoma
Keyword: Genetics,
Phytopathology,
Fungi,
Pseudocercospora ulei,
Natural rubber,
Hevea brasiliensis,
Genome
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