Functional analysis of alternative castor bean DGAT enzymes
Document title: Functional analysis of alternative castor bean DGAT enzymes
Journal: Genetics and molecular biology
Database: PERIÓDICA
System number: 000459145
ISSN: 1415-4757
Authors: 1
2
1
1
2
Institutions: 1Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul. Brasil
Year:
Volumen: 46
Number: 1
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, analítico
English abstract The diversity of diacylglycerol acyltransferases (DGATs) indicates alternative roles for these enzymes in plant metabolism besides triacylglycerol (TAG) biosynthesis. In this work, we functionally characterized castor bean (Ricinus communis L.) DGATs assessing their subcellular localization, expression in seeds, capacity to restore triacylglycerol (TAG) biosynthesis in mutant yeast and evaluating whether they provide tolerance over free fatty acids (FFA) in sensitive yeast. RcDGAT3 displayed a distinct subcellular localization, located in vesicles outside the endoplasmic reticulum (ER) in most leaf epidermal cells. This enzyme was unable to restore TAG biosynthesis in mutant yeast; however, it was able to outperform other DGATs providing higher tolerance over FFA. RcDAcTA subcellular localization was associated with the ER membranes, resembling RcDGAT1 and RcDGAT2, but it failed to rescue the long-chain TAG biosynthesis in mutant yeast, even with fatty acid supplementation. Besides TAG biosynthesis, our results suggest that RcDGAT3 might have alternative functions and roles in lipid metabolism
Disciplines: Biología,
Química
Keyword: Angiospermas,
Fitoquímica,
Ricinus communis,
Enzimas,
Análisis funcional
Keyword: Angiosperms,
Phytochemistry,
Ricinus communis,
Enzymes,
Functional analysis
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