Functional analysis of alternative castor bean DGAT enzymes
Título del documento: Functional analysis of alternative castor bean DGAT enzymes
Revista: Genetics and molecular biology
Base de datos: PERIÓDICA
Número de sistema: 000459145
ISSN: 1415-4757
Autores: 1
2
1
1
2
Instituciones: 1Universidade Federal do Rio Grande do Sul, Centro de Biotecnologia, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul. Brasil
Año:
Volumen: 46
Número: 1
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés The diversity of diacylglycerol acyltransferases (DGATs) indicates alternative roles for these enzymes in plant metabolism besides triacylglycerol (TAG) biosynthesis. In this work, we functionally characterized castor bean (Ricinus communis L.) DGATs assessing their subcellular localization, expression in seeds, capacity to restore triacylglycerol (TAG) biosynthesis in mutant yeast and evaluating whether they provide tolerance over free fatty acids (FFA) in sensitive yeast. RcDGAT3 displayed a distinct subcellular localization, located in vesicles outside the endoplasmic reticulum (ER) in most leaf epidermal cells. This enzyme was unable to restore TAG biosynthesis in mutant yeast; however, it was able to outperform other DGATs providing higher tolerance over FFA. RcDAcTA subcellular localization was associated with the ER membranes, resembling RcDGAT1 and RcDGAT2, but it failed to rescue the long-chain TAG biosynthesis in mutant yeast, even with fatty acid supplementation. Besides TAG biosynthesis, our results suggest that RcDGAT3 might have alternative functions and roles in lipid metabolism
Disciplinas: Biología,
Química
Palabras clave: Angiospermas,
Fitoquímica,
Ricinus communis,
Enzimas,
Análisis funcional
Keyword: Angiosperms,
Phytochemistry,
Ricinus communis,
Enzymes,
Functional analysis
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