Protocol for regeneration in vitro of Arachis hypogaea L



Document title: Protocol for regeneration in vitro of Arachis hypogaea L
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000267197
ISSN: 0717-3458
Authors: 1
Institutions: 1Universidad Nacional de Río Cuarto, Departamento de Microbiología e Inmunología, Río Cuarto, Córdoba. Argentina
Year:
Season: Ago
Volumen: 3
Number: 2
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage
Disciplines: Agrociencias
Keyword: Plantas para uso industrial,
Biotecnología,
Genética,
Arachis hypogaea,
Cacahuate,
Reguladores del crecimiento,
Embriogénesis somática,
Herbicidas,
Resistencia
Keyword: Agricultural sciences,
Plants for industrial use,
Arachis hypogaea,
Growth regulators,
Peanut,
Herbicides,
Somatic embryogenesis,
Resistance,
Genetics
Full text: Texto completo (Ver HTML)