Protocol for regeneration in vitro of Arachis hypogaea L



Título del documento: Protocol for regeneration in vitro of Arachis hypogaea L
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000267197
ISSN: 0717-3458
Autores: 1
Instituciones: 1Universidad Nacional de Río Cuarto, Departamento de Microbiología e Inmunología, Río Cuarto, Córdoba. Argentina
Año:
Periodo: Ago
Volumen: 3
Número: 2
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The regeneration of peanut Runner varieties grown in the South of the province of Cordoba, Argentina, was studied to count on a regeneration protocol, which is essential to perform genetic transformation of the plant. The induction of somatic embryos was evaluated in different peanut explants cultivated in Cordoba: cotyledons with and without embryos, epycotils and leaflets, of the Tegua, Nahuel and Florman INTA varieties. The induction medium consisted of MS salts with 30 g.l-1 sucrose, supplemented with the following hormones and herbicides: BAP, Picloram, DMA and 2,4-D. The highest percentage of embryogenic callus was produced cultivating epicotyls with 50 mg.l-1 2,4-D. The embryos obtained produced plantlets which, once acclimatized, reached the reproductive stage
Disciplinas: Agrociencias
Palabras clave: Plantas para uso industrial,
Biotecnología,
Genética,
Arachis hypogaea,
Cacahuate,
Reguladores del crecimiento,
Embriogénesis somática,
Herbicidas,
Resistencia
Keyword: Agricultural sciences,
Plants for industrial use,
Arachis hypogaea,
Growth regulators,
Peanut,
Herbicides,
Somatic embryogenesis,
Resistance,
Genetics
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