| Journal: | Electronic journal of biotechnology |
| Database: | PERIÓDICA |
| System number: | 000358916 |
| ISSN: | 0717-3458 |
| Authors: | Cao, Ying1 Hu, Shang-lian1 Huang, Sheng-xiong1 Ren, Peng1 Lu, Xue-qin1 |
| Institutions: | 1Southwest University of Science and Technology, College of Life Science and Engineering, Mianyang. China |
| Year: | 2012 |
| Volumen: | 15 |
| Number: | 5 |
| Country: | Chile |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, analítico |
| English abstract | 4-coumarate:CoA ligase (4CL) plays an important role at the divergence point from general phenylpropanoid metabolism to several branch pathways. Although 4CL sin higher plants have been extensively studied, little has known about the 4CL gene of bamboo. Results: In current study, a Na4CL gene putative encoding 4-coumarate:CoA ligase (4CL) and its 5-flanking region were isolated from bamboo (Neosinocalamus affinis) by RACE-PCR and genomic DNA walker, respectively. Na4CL encodes a predicted protein of 557 amino acids, with conserved motifs of adenylate-forming enzymes. Phylogenetic analysis showed that Na4CL shared 62~85% identity with other known plant 4CLs, and cluster closely with some known 4CLs in monocots. Sequence analysis revealed conserved cis-acting elements (Box A and AC-II element) present in the Na4CL promoter. Additionally, a Na4CL RNAi construct was transformed into tobacco. Transgenic tobaccos displayed significant down-expression of endogenesis 4CL and reduced lignin contents. Conclusion: These results contribute to the knowledge of the presence of Na4CL gene and its possible role in phenylpropanoid metabolism |
| Disciplines: | Agrociencias, Biología |
| Keyword: | Plantas para uso industrial, Fisiología vegetal, Genética, Biología molecular, Biotecnología, Bambú, Neosinocalamus affinis, Expresión génica, Clonación |
| Keyword: | Agricultural sciences, Biology, Plants for industrial use, Genetics, Plant physiology, Molecular biology, Biotechnology, Bamboo, Neosinocalamus affinis, Genetic expression, Cloning |
| Full text: | Texto completo (Ver HTML) |
