Molecular cloning, expression pattern, and putative cis-acting elements of a 4-coumarate:CoA ligase gene in bamboo (Neosinocalamus affinis)
Título del documento: Molecular cloning, expression pattern, and putative cis-acting elements of a 4-coumarate:CoA ligase gene in bamboo (Neosinocalamus affinis)
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000358916
ISSN: 0717-3458
Autores: 1
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Instituciones: 1Southwest University of Science and Technology, College of Life Science and Engineering, Mianyang. China
Año:
Volumen: 15
Número: 5
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés 4-coumarate:CoA ligase (4CL) plays an important role at the divergence point from general phenylpropanoid metabolism to several branch pathways. Although 4CL sin higher plants have been extensively studied, little has known about the 4CL gene of bamboo. Results: In current study, a Na4CL gene putative encoding 4-coumarate:CoA ligase (4CL) and its 5’-flanking region were isolated from bamboo (Neosinocalamus affinis) by RACE-PCR and genomic DNA walker, respectively. Na4CL encodes a predicted protein of 557 amino acids, with conserved motifs of adenylate-forming enzymes. Phylogenetic analysis showed that Na4CL shared 62~85% identity with other known plant 4CLs, and cluster closely with some known 4CLs in monocots. Sequence analysis revealed conserved cis-acting elements (Box A and AC-II element) present in the Na4CL promoter. Additionally, a Na4CL RNAi construct was transformed into tobacco. Transgenic tobaccos displayed significant down-expression of endogenesis 4CL and reduced lignin contents. Conclusion: These results contribute to the knowledge of the presence of Na4CL gene and its possible role in phenylpropanoid metabolism
Disciplinas: Agrociencias,
Biología
Palabras clave: Plantas para uso industrial,
Fisiología vegetal,
Genética,
Biología molecular,
Biotecnología,
Bambú,
Neosinocalamus affinis,
Expresión génica,
Clonación
Keyword: Agricultural sciences,
Biology,
Plants for industrial use,
Genetics,
Plant physiology,
Molecular biology,
Biotechnology,
Bamboo,
Neosinocalamus affinis,
Genetic expression,
Cloning
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