Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)



Document title: Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000357879
ISSN: 0717-3458
Authors: 1
1
1
1
Institutions: 1Yangzhou University, College of Horticulture and Plant Protection, Yangzhou. China
Year:
Volumen: 15
Number: 6
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering
Disciplines: Biología
Keyword: Biotecnología,
Biosíntesis,
Antocianinas,
Expresión génica,
Glucosiltransferasa,
Paeonia lactiflora,
Paeoniaceae
Keyword: Biology,
Biotechnology,
Biosynthesis,
Anthocyanins,
Gene expression,
Glucosyltransferase,
Paeonia lactiflora,
Paeoniaceae
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