Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
Título del documento: Isolation of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene and expression analysis of anthocyanin biosynthetic genes in herbaceous peony (Paeonia lactiflora Pall.)
Revue: Electronic journal of biotechnology
Base de datos: PERIÓDICA
Número de sistema: 000357879
ISSN: 0717-3458
Autores: 1
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1
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Instituciones: 1Yangzhou University, College of Horticulture and Plant Protection, Yangzhou. China
Año:
Volumen: 15
Número: 6
País: Chile
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés Herbaceous peony (Paeonia lactiflora Pall.) is an excellent material for studying the formation of flower colour because of its abundant colour. The full-length cDNA of a UDP-glucose: Flavonoid 5-O-glucosyltransferase gene (UF5GT) containing 1629 bp nucleotides was obtained from P. lactiflora. The expression patterns of nine related anthocyanin biosynthetic genes (PlPSY, PlCHS, PlCHI, PlF3H, PlF3'H, PlDFR, PlANS, PlUF3GT and PlUF5GT) in diurnal variation petals showed that their expression peaks were basically at 15:00 and the expression patterns were consistent with the trend of sampling conditions except individual gene. And the highest expression levels were in PlCHS, PlDFR and PlUF3GT, which could be the candidates to regulate P. lactiflora flower colour by means of genetic engineering
Disciplinas: Biología
Palabras clave: Biotecnología,
Biosíntesis,
Antocianinas,
Expresión génica,
Glucosiltransferasa,
Paeonia lactiflora,
Paeoniaceae
Keyword: Biology,
Biotechnology,
Biosynthesis,
Anthocyanins,
Gene expression,
Glucosyltransferase,
Paeonia lactiflora,
Paeoniaceae
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