Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain
Document title: Improved isolation of good-quality total RNA from the optic stalk of Mud crab, Scylla paramamosain
Journal: Electronic journal of biotechnology
Database: PERIÓDICA
System number: 000358462
ISSN: 0717-3458
Authors: 1
2
2
2
1
2
Institutions: 1Fudan University, School of Life Sciences, Shanghai. China
2Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute, Shanghai. China
Year:
Volumen: 15
Number: 2
Country: Chile
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified protocol and three conventional methods were also employed to confirm the quality of RNA. This improved method would be helpful in facilitating molecular research of crabs involving RNA from the optic stalk
Disciplines: Biología,
Química
Keyword: Crustáceos,
Bioquímica,
Biotecnología,
Aislamiento químico,
ARN,
Pedúnculo ocular,
Acetato de potasio,
Scylla paramamosain
Keyword: Biology,
Chemistry,
Crustaceans,
Biochemistry,
Biotechnology,
Chemical isolation,
Eyestalk,
RNA,
Potassium acetate,
Scylla paramamosain
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