| Revue: | Electronic journal of biotechnology |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000358462 |
| ISSN: | 0717-3458 |
| Autores: | Zhu, Houze1 Jiang, Keji2 Zhang, Fengying2 Zhang, Dan2 Pi, Yan1 Ma, Lingbo2 |
| Instituciones: | 1Fudan University, School of Life Sciences, Shanghai. China 2Chinese Academy of Fishery Sciences, East China Sea Fisheries Research Institute, Shanghai. China |
| Año: | 2012 |
| Volumen: | 15 |
| Número: | 2 |
| País: | Chile |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, aplicado |
| Resumen en inglés | An improved and efficient protocol was developed based on the TaKaRa RNAiso Plus Kit (Code: D9108A) for isolating good-quality total RNA from the optic stalk of mud crab, Scylla paramamosain. The protocol was based on the Trizol method with modifications. The carapace overlapping the optic stalk was retained with RNA in regular protocol. In order to remove the abundant deposition correlative with the carapace which makes the isolation of RNA particularly difficult, 5M potassium acetate solution (pH = 6.0) was added before the precipitation of RNA, and the temperature of RNA deposition was also decreased to -70ºC to ensure the stabilization of RNA. Good-quality total RNA from the optic stalk of S. paramamosain could be easily isolated with this modified protocol and three conventional methods were also employed to confirm the quality of RNA. This improved method would be helpful in facilitating molecular research of crabs involving RNA from the optic stalk |
| Disciplinas: | Biología, Química |
| Palabras clave: | Crustáceos, Bioquímica, Biotecnología, Aislamiento químico, ARN, Pedúnculo ocular, Acetato de potasio, Scylla paramamosain |
| Keyword: | Biology, Chemistry, Crustaceans, Biochemistry, Biotechnology, Chemical isolation, Eyestalk, RNA, Potassium acetate, Scylla paramamosain |
| Texte intégral: | Texto completo (Ver HTML) |
