| Journal: | Brazilian Journal of Pharmaceutical Sciences |
| Database: | PERIÓDICA |
| System number: | 000451911 |
| ISSN: | 1984-8250 |
| Authors: | Tundisi, Louise Lacalendola1 Coelho, Diego de Freitas2 Faria, Alessandra Valéria de Sousa1 Pessoa, Adalberto3 Tambourgi, Elias Basile2 Nascimento, Laura de Oliveira4 Silveira, Edgar5 Mazzola, Priscila Gava4 |
| Institutions: | 1Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil 2Universidade Estadual de Campinas, Faculdade de Engenharia Química, Campinas, Sao Paulo. Brasil 3Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil 4Universidade Estadual de Campinas, Faculdade de Ciencias Farmaceuticas, Campinas, Sao Paulo. Brasil 5Universidade Federal de Uberlandia, Instituto de Genetica e Bioquimica, Uberlandia, Minas Gerais. Brasil |
| Year: | 2022 |
| Volumen: | 58 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients’ chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed |
| Disciplines: | Química |
| Keyword: | Química farmacéutica, Purificación química, L-asparaginasa, Sistema acuoso bifásico, Procesos derivados, Bioprocesamiento, Precipitación fraccionada |
| Keyword: | Medicinal chemistry, Chemical purification, L-asparaginase, Aqueous two-phase system, Downstream processes, Bioprocessing, Fractional precipitation |
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