Two-Step Purification of L-Asparaginase from Acrylaway® L



Título del documento: Two-Step Purification of L-Asparaginase from Acrylaway® L
Revista: Brazilian Journal of Pharmaceutical Sciences
Base de datos: PERIÓDICA
Número de sistema: 000451911
ISSN: 1984-8250
Autors: 1
2
1
3
2
4
5
4
Institucions: 1Universidade Estadual de Campinas, Instituto de Biologia, Campinas, Sao Paulo. Brasil
2Universidade Estadual de Campinas, Faculdade de Engenharia Química, Campinas, Sao Paulo. Brasil
3Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Sao Paulo. Brasil
4Universidade Estadual de Campinas, Faculdade de Ciencias Farmaceuticas, Campinas, Sao Paulo. Brasil
5Universidade Federal de Uberlandia, Instituto de Genetica e Bioquimica, Uberlandia, Minas Gerais. Brasil
Any:
Volum: 58
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés L-Asparaginase (L-ASNase) is a biopharmaceutical used for acute lymphoblastic leukaemia (ALL) treatment, dramatically increasing the patients’ chance of cure. However, its production and distribution in developing countries were disrupted because of its low profitability, which caused great concern among patients. This study evaluates the feasibility of combining fractional precipitation and aqueous two-phase systems (ATPS) to purify L-ASNase from a low-grade product, commercially known as Acrylaway® L. The ATPS purification results were not particularly expressive compared to the two-step purification process composed of ethanol precipitation and gel filtration, which was able to recover the target molecule with a purification factor over 5 fold. Thus, we studied a purification process capable of manufacturing pharmaceutical grade L-ASNase from a commercially available low-grade raw material; however, improvements regarding its throughput must be achieved, and high purity is the first step to apply it as a new biopharmaceutical product. The proposed process could pose as a short-time solution to mitigate its shortage while a cost-effective production plant is being developed
Disciplines Química
Paraules clau: Química farmacéutica,
Purificación química,
L-asparaginasa,
Sistema acuoso bifásico,
Procesos derivados,
Bioprocesamiento,
Precipitación fraccionada
Keyword: Medicinal chemistry,
Chemical purification,
L-asparaginase,
Aqueous two-phase system,
Downstream processes,
Bioprocessing,
Fractional precipitation
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