Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate



Document title: Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate
Journal: Brazilian journal of chemical engineering
Database: PERIÓDICA
System number: 000308605
ISSN: 0104-6632
Authors: 1
2
3
Institutions: 1Faculdade de Engenharia Quimica de Lorena, Departamento de Biotecnologia, Lorena, Sao Paulo. Brasil
2Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Araraquara, Sao Paulo. Brasil
3Universidade de Sao Paulo, Escola Politecnica, Sao Paulo. Brasil
Year:
Season: Sep
Volumen: 15
Number: 3
Pages: 273-279
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract A eucalyptus hemicellulose fraction was hydrolysed by treating eucalyptus wood chips with sulfuric acid. The hydrolysate was used as the substrate to grow Paecilomyces variotii IOC-3764 cultured for 72 or 96 hours. The influence of the inhibitors, nutrients and fermentation time was verified by a 28-4 and, subsequently, a 25-1 fractional factorial design. The effects of the inhibitors (acetic acid and furfural), nutrients (rice bran, urea, potassium nitrate, ammonium sulfate, magnesium sulfate and sodium phosphate) and fermentation time were investigated. The highest yield (10.59 g/L of biomass) was obtained when the microorganisms were cultivated for 72 hours in a medium composed of 30 g/L rice bran, 9.4 g/L ammonium sulfate (2 g/L nitrogen) and 2 g/L sodium phosphate
Disciplines: Química
Keyword: Fermentaciones,
Sustratos experimentales,
Eucalipto,
Producción de biomasa,
Paecilomyces variotii
Keyword: Chemistry,
Fermentation,
Experimental substrates,
Eucalyptus,
Biomass production,
Paecilomyces variotii
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