Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate



Título del documento: Use of fractional factorial design for selection of nutrients for culturing Paecilomyces variotii in eucalyptus hemicellulosic hydrolysate
Revista: Brazilian journal of chemical engineering
Base de datos: PERIÓDICA
Número de sistema: 000308605
ISSN: 0104-6632
Autores: 1
2
3
Instituciones: 1Faculdade de Engenharia Quimica de Lorena, Departamento de Biotecnologia, Lorena, Sao Paulo. Brasil
2Universidade de Sao Paulo, Faculdade de Ciencias Farmaceuticas, Araraquara, Sao Paulo. Brasil
3Universidade de Sao Paulo, Escola Politecnica, Sao Paulo. Brasil
Año:
Periodo: Sep
Volumen: 15
Número: 3
Paginación: 273-279
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés A eucalyptus hemicellulose fraction was hydrolysed by treating eucalyptus wood chips with sulfuric acid. The hydrolysate was used as the substrate to grow Paecilomyces variotii IOC-3764 cultured for 72 or 96 hours. The influence of the inhibitors, nutrients and fermentation time was verified by a 28-4 and, subsequently, a 25-1 fractional factorial design. The effects of the inhibitors (acetic acid and furfural), nutrients (rice bran, urea, potassium nitrate, ammonium sulfate, magnesium sulfate and sodium phosphate) and fermentation time were investigated. The highest yield (10.59 g/L of biomass) was obtained when the microorganisms were cultivated for 72 hours in a medium composed of 30 g/L rice bran, 9.4 g/L ammonium sulfate (2 g/L nitrogen) and 2 g/L sodium phosphate
Disciplinas: Química
Palabras clave: Fermentaciones,
Sustratos experimentales,
Eucalipto,
Producción de biomasa,
Paecilomyces variotii
Keyword: Chemistry,
Fermentation,
Experimental substrates,
Eucalyptus,
Biomass production,
Paecilomyces variotii
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