| Journal: | Brazilian archives of biology and technology |
| Database: | PERIÓDICA |
| System number: | 000387745 |
| ISSN: | 1516-8913 |
| Authors: | Valle, Gabrielle M1 Cinelli, Leonardo P2 Todeschini, Adriane R3 Brito Gitirana, Lycia de4 Vilela Silva, Ana Cristina E.S1 Mourao, Paulo A.S1 |
| Institutions: | 1Universidade Federal do Rio de Janeiro, Hospital Universitario Clementino Fraga Filho, Rio de Janeiro. Brasil 2Universidade Federal do Rio de Janeiro, Laboratorio Integrado de Prospeccao em Produtos Bioativos, Macae, Rio de Janeiro. Brasil 3Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil 4Universidade Federal do Rio de Janeiro, Instituto de Ciencias Biomedicas, Rio de Janeiro. Brasil |
| Year: | 2015 |
| Season: | Jul-Ago |
| Volumen: | 58 |
| Number: | 4 |
| Pages: | 617-627 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental, aplicado |
| English abstract | This work found the occurrence of a distinct sialic acid-rich polysaccharide in the sperm surface of the sea urchin Lytechinus variegatus, which differed significantly from a similar molecule found in the egg jelly. The sperm polysaccharide extracted by protease digestion was purified using anion exchange chromatography and characterized using agarose gel electrophoresis, gas chromatography/mass spectrometry and NMR spectroscopy. This polysaccharide was highly sulfated and composed almost exclusively of N-acetylneuraminic acid. In contrast, the sialic acid-rich polysaccharide from the egg jelly was composed of N-glycolylneuraminic acid and contains several other hexoses in its structure. This new molecule could help to characterize in further detail the mechanism of fertilization in the sea urchin model system. Sulfated polysaccharides from the jelly coat of sea urchins showed species-specificity in inducing the sperm acrosome reaction, providing an example of a signal transduction event regulated by the sulfated polysaccharide. The new sialic acid-rich polysaccharide found in the sperm head could represent a new molecule involved in the biology of the sea urchin fertilization |
| Disciplines: | Biología, Química |
| Keyword: | Invertebrados, Bioquímica, Erizos de mar, Esperma, Acido siálico, Polisacáridos sulfatados, Lytechinus variegatus, Toxopneustidae |
| Keyword: | Biology, Chemistry, Invertebrates, Biochemistry, Sea urchins, Sperm, Sialic acid, Sulfated polysaccharides, Lytechinus variegatus, Toxopneustidae |
| Full text: | Texto completo (Ver HTML) |
