Sperm and Egg Jelly Coat from Sea Urchin Lytechinus variegatus Collected in Rio de Janeiro Contain Distinct Sialic Acid-Rich Polysaccharides



Título del documento: Sperm and Egg Jelly Coat from Sea Urchin Lytechinus variegatus Collected in Rio de Janeiro Contain Distinct Sialic Acid-Rich Polysaccharides
Revista: Brazilian archives of biology and technology
Base de datos: PERIÓDICA
Número de sistema: 000387745
ISSN: 1516-8913
Autores: 1
2
3
4
1
1
Instituciones: 1Universidade Federal do Rio de Janeiro, Hospital Universitario Clementino Fraga Filho, Rio de Janeiro. Brasil
2Universidade Federal do Rio de Janeiro, Laboratorio Integrado de Prospeccao em Produtos Bioativos, Macae, Rio de Janeiro. Brasil
3Universidade Federal do Rio de Janeiro, Instituto de Biofisica Carlos Chagas Filho, Rio de Janeiro. Brasil
4Universidade Federal do Rio de Janeiro, Instituto de Ciencias Biomedicas, Rio de Janeiro. Brasil
Año:
Periodo: Jul-Ago
Volumen: 58
Número: 4
Paginación: 617-627
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés This work found the occurrence of a distinct sialic acid-rich polysaccharide in the sperm surface of the sea urchin Lytechinus variegatus, which differed significantly from a similar molecule found in the egg jelly. The sperm polysaccharide extracted by protease digestion was purified using anion exchange chromatography and characterized using agarose gel electrophoresis, gas chromatography/mass spectrometry and NMR spectroscopy. This polysaccharide was highly sulfated and composed almost exclusively of N-acetylneuraminic acid. In contrast, the sialic acid-rich polysaccharide from the egg jelly was composed of N-glycolylneuraminic acid and contains several other hexoses in its structure. This new molecule could help to characterize in further detail the mechanism of fertilization in the sea urchin model system. Sulfated polysaccharides from the jelly coat of sea urchins showed species-specificity in inducing the sperm acrosome reaction, providing an example of a signal transduction event regulated by the sulfated polysaccharide. The new sialic acid-rich polysaccharide found in the sperm head could represent a new molecule involved in the biology of the sea urchin fertilization
Disciplinas: Biología,
Química
Palabras clave: Invertebrados,
Bioquímica,
Erizos de mar,
Esperma,
Acido siálico,
Polisacáridos sulfatados,
Lytechinus variegatus,
Toxopneustidae
Keyword: Biology,
Chemistry,
Invertebrates,
Biochemistry,
Sea urchins,
Sperm,
Sialic acid,
Sulfated polysaccharides,
Lytechinus variegatus,
Toxopneustidae
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