Journal: | Biocell |
Database: | PERIÓDICA |
System number: | 000320485 |
ISSN: | 0327-9545 |
Authors: | Lin, Juan1 Zhang, Wen1 Shi, Mingzhu1 Wang, Xinglong1 Sun, Xiaofen1 Tang, Kexuan2 |
Institutions: | 1Fudan University, School of Life Sciences, Shanghai. China 2Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai. China |
Year: | 2008 |
Season: | Dic |
Volumen: | 32 |
Number: | 3 |
Pages: | 229-235 |
Country: | Argentina |
Language: | Inglés |
Document type: | Artículo |
Approach: | Experimental, aplicado |
English abstract | A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt |
Disciplines: | Química, Biología |
Keyword: | Bioquímica, Fisiología vegetal, Genética, Genes, Caracterización bioquímica, Intercambio catiónico, Capsella bursa-pastoris |
Keyword: | Chemistry, Biology, Biochemistry, Genetics, Plant physiology, Genes, Biochemical characterization, Cationic exchange, Capsella bursa-pastoris |
Full text: | Texto completo (Ver HTML) |