Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris



Título del documento: Isolation and molecular characterization of a cax gene from Capsella bursa-pastoris
Revista: Biocell
Base de datos: PERIÓDICA
Número de sistema: 000320485
ISSN: 0327-9545
Autores: 1
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2
Instituciones: 1Fudan University, School of Life Sciences, Shanghai. China
2Shanghai Jiaotong University, School of Agriculture and Biology, Shanghai. China
Año:
Periodo: Dic
Volumen: 32
Número: 3
Paginación: 229-235
País: Argentina
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés A new cation exchangers (CAXs) gene was cloned and characterized from Capsella bursapastoris by rapid amplification of cDNA ends (RACE). The full-length cDNA sequence of cax from C. bursa-pastoris (designated as Cbcax51) was 1754 bp containing a 1398 bp open reading frame encoding a polypeptide of 466 amino-acid residues with a calculated molecular mass of 50.5 kDa and an isoelectric point of 5.69. The predicted CbCAX51 contained an IMP dehydrogenase/GMP reductase domain, two Na+/Ca2+ exchanger protein domains. Comparative and bioinformatics analyses revealed that CbCAX51 showed extensive homology with CAX from other plant species. The expression analysis by different treatments indicated that Cbcax51 could be activated by cold triggering and was related to the cold acclimation process, but its expression is regulated negatively by drought and not affected by ABA or salt
Disciplinas: Química,
Biología
Palabras clave: Bioquímica,
Fisiología vegetal,
Genética,
Genes,
Caracterización bioquímica,
Intercambio catiónico,
Capsella bursa-pastoris
Keyword: Chemistry,
Biology,
Biochemistry,
Genetics,
Plant physiology,
Genes,
Biochemical characterization,
Cationic exchange,
Capsella bursa-pastoris
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