| Journal: | Anais da Academia Brasileira de Ciencias |
| Database: | PERIÓDICA |
| System number: | 000436167 |
| ISSN: | 0001-3765 |
| Authors: | Patarroyo Vargas, Adriana M1 Cordeiro, Gláucia1 Silva, Carolina R. da1 Silva, Camila R. da2 Mendonca, Eduardo G1 Visotto, Liliane E3 Zanuncio, José C1 Campos, Wellington G4 Oliveira, Maria Goreti A1 |
| Institutions: | 1Universidade Federal de Vicosa, Instituto de Biotecnologia Aplicada a Agropecuaria, Vicosa, Minas Gerais. Brasil 2Universidade Federal de Vicosa, Instituto de Ciencias Agrarias, Rio Paranaiba, Minas Gerais. Brasil 3Universidade Federal de Vicosa, Instituto de Ciencias Biologicas, Rio Paranaiba, Minas Gerais. Brasil 4Universidade Federal de Sao Joao del Rei, Departamento de Engenharia de Biossistemas, Sao Joao del Rei, Minas Gerais. Brasil |
| Year: | 2020 |
| Volumen: | 92 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Aplicado, descriptivo |
| English abstract | Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors |
| Disciplines: | Biología |
| Keyword: | Insectos, Bioquímica, Proteasas, Cinética enzimática, Inhibidores competitivos, Anticarsia gemmatalis, Lepidoptera |
| Keyword: | Insects, Biochemistry, Proteases, Enzymatic kinetics, Competitive inhibitors, Anticarsia gemmatalis, Lepidoptera |
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