Inhibition kinetics of digestive proteases for Anticarsia gemmatalis



Título del documento: Inhibition kinetics of digestive proteases for Anticarsia gemmatalis
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000436167
ISSN: 0001-3765
Autores: 1
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Instituciones: 1Universidade Federal de Vicosa, Instituto de Biotecnologia Aplicada a Agropecuaria, Vicosa, Minas Gerais. Brasil
2Universidade Federal de Vicosa, Instituto de Ciencias Agrarias, Rio Paranaiba, Minas Gerais. Brasil
3Universidade Federal de Vicosa, Instituto de Ciencias Biologicas, Rio Paranaiba, Minas Gerais. Brasil
4Universidade Federal de Sao Joao del Rei, Departamento de Engenharia de Biossistemas, Sao Joao del Rei, Minas Gerais. Brasil
Año:
Volumen: 92
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Aplicado, descriptivo
Resumen en inglés Anticarsia gemmatalis Hübner, 1818 (Lepidoptera) is a major pest of soybean in the Brazil. It is known that the reduction of proteolytic activity by the ingestion of protease inhibitors reduces digestion and larval development of the insects. Control via inhibition of the digestive enzymes necessitates deeper knowledge of the enzyme kinetics and the characterization of the inhibition kinetics of these proteases, for better understanding of the active centers and action mechanisms of this enzyme. Trypsin-like proteases found in the gut of Anticarsia gemmatalis were purified in a p-aminobenzamidine agarose column. Kinetic characterization showed KM 0.503 mM for the L-BApNA substrate; Vmax= 46.650 nM s-1; Vmax/[E]= 9.256 nM s-1 mg L-1 and Vmax/[E]/KM= 18.402 nM s-1 mg L-1 mM. The Ki values for the inhibitors benzamidine, berenil, SKTI and SBBI were 11.2 µM, 32.4 µM, 0.25 nM and 1.4 nM, respectively, and all revealed linear competitive inhibition. The SKTI showed the greatest inhibition, which makes it a promising subject for future research to manufacture peptide mimetic inhibitors
Disciplinas: Biología
Palabras clave: Insectos,
Bioquímica,
Proteasas,
Cinética enzimática,
Inhibidores competitivos,
Anticarsia gemmatalis,
Lepidoptera
Keyword: Insects,
Biochemistry,
Proteases,
Enzymatic kinetics,
Competitive inhibitors,
Anticarsia gemmatalis,
Lepidoptera
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