Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Document title: Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000409001
ISSN: 0001-3765
Authors: 1
1
1
1
2
3
Institutions: 1Universidade Federal do Parana, Departamento de Zoologia, Curitiba, Parana. Brasil
2Universidade Federal do Parana, Departamento de Bioquimica, Curitiba, Parana. Brasil
3Universidade Federal do Parana, Departamento de Zootecnia, Curitiba, Parana. Brasil
Year:
Season: Jun
Volumen: 89
Number: 2
Pages: 1041-1046
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range
Disciplines: Biología
Keyword: Ecología,
Moluscos,
Especies invasoras,
ADN,
Identificación molecular,
Muestreo ecológico,
Limnosperma fortunei,
Mytilidae
Keyword: Biology,
Ecology,
Molluscs,
Invasive species,
DNA,
Molecular identification,
Ecological sampling,
Limnosperma fortunei,
Mytilidae
Full text: Texto completo (Ver HTML)