Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Título del documento: Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei , Mytilidae) in environmental samples
Revista: Anais da Academia Brasileira de Ciencias
Base de datos: PERIÓDICA
Número de sistema: 000409001
ISSN: 0001-3765
Autores: 1
1
1
1
2
3
Instituciones: 1Universidade Federal do Parana, Departamento de Zoologia, Curitiba, Parana. Brasil
2Universidade Federal do Parana, Departamento de Bioquimica, Curitiba, Parana. Brasil
3Universidade Federal do Parana, Departamento de Zootecnia, Curitiba, Parana. Brasil
Año:
Periodo: Jun
Volumen: 89
Número: 2
Paginación: 1041-1046
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, aplicado
Resumen en inglés The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range
Disciplinas: Biología
Palabras clave: Ecología,
Moluscos,
Especies invasoras,
ADN,
Identificación molecular,
Muestreo ecológico,
Limnosperma fortunei,
Mytilidae
Keyword: Biology,
Ecology,
Molluscs,
Invasive species,
DNA,
Molecular identification,
Ecological sampling,
Limnosperma fortunei,
Mytilidae
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