Revista: | Anais da Academia Brasileira de Ciencias |
Base de datos: | PERIÓDICA |
Número de sistema: | 000409001 |
ISSN: | 0001-3765 |
Autors: | Pie, Marcio R1 Stroher, Patricia R1 Agostinis, Andre O1 Belmonte-Lopes, Ricardo1 Tadra-Sfeir, Michelle Z2 Ostrensky, Antonio3 |
Institucions: | 1Universidade Federal do Parana, Departamento de Zoologia, Curitiba, Parana. Brasil 2Universidade Federal do Parana, Departamento de Bioquimica, Curitiba, Parana. Brasil 3Universidade Federal do Parana, Departamento de Zootecnia, Curitiba, Parana. Brasil |
Any: | 2017 |
Període: | Jun |
Volum: | 89 |
Número: | 2 |
Paginació: | 1041-1046 |
País: | Brasil |
Idioma: | Inglés |
Tipo de documento: | Artículo |
Enfoque: | Experimental, aplicado |
Resumen en inglés | The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range |
Disciplines | Biología |
Paraules clau: | Ecología, Moluscos, Especies invasoras, ADN, Identificación molecular, Muestreo ecológico, Limnosperma fortunei, Mytilidae |
Keyword: | Biology, Ecology, Molluscs, Invasive species, DNA, Molecular identification, Ecological sampling, Limnosperma fortunei, Mytilidae |
Text complet: | Texto completo (Ver HTML) |