Antineoplastic potential and chemical evaluation of essential oils from leaves and flowers of Tagetes ostenii Hicken
Document title: Antineoplastic potential and chemical evaluation of essential oils from leaves and flowers of Tagetes ostenii Hicken
Journal: Anais da Academia Brasileira de Ciencias
Database: PERIÓDICA
System number: 000435967
ISSN: 0001-3765
Authors: 1
1
2
1
1
3
1
Institutions: 1Instituto Federal de Educacao, Ciencia e Tecnologia do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul. Brasil
2Universidade Federal do Rio Grande do Sul, Faculdade de Farmacia, Porto Alegre, Rio Grande do Sul. Brasil
3Universidade Federal de Ciencias da Saude de Porto Alegre, Porto Alegre, Rio Grande do Sul. Brasil
Year:
Volumen: 92
Country: Brasil
Language: Inglés
Document type: Artículo
Approach: Experimental, aplicado
English abstract Breast and cervical cancer represent a major problem of women’s global public health. Here, we investigated the chemical composition of essential oils from leaves and flowers of T. ostenii and the antineoplastic potential in a cervical cancer and breast cancer cell line, SiHa and MCF-7; and non-tumoral cells, HaCat. The chemical analysis revealed a predominance of oxygenated monoterpenes in both essential oils. The IC50 after 24 h of treatment was 72 ng/mL for EO 1 and 83 ng/mL for EO 2 in SiHa cells. For MCF-7 the IC50 was 174.3 ng/mL for EO 1. For HaCat cells it was 54.45 ng/mL for EO 1 and 20.83 ng/mL for EO 2. A synergistic effect with both essential oils and cisplatin was also verified. SiHa cells had their wound healing capacity reduced after 48 h of treatment with EO 2 and both essential oils were able to significantly inhibit the adhesion process and clonogenic ability after 24 h of treatment. Our results suggest a long-lasting inhibitory activity in SiHa cells because of the low recovery capacity of cells after treatment withdrawal. Flow cytometer with annexin V/propidium iodide demonstrated a majority cell death through late apoptosis after 24, 48 and 72 hours of treatment
Disciplines: Medicina,
Química
Keyword: Fitoquímica,
Farmacología,
Oncología,
Aceites esenciales,
Composición química,
Actividad antineoplásica,
Cáncer cervical,
Citotoxicidad,
Tagetes ostenii,
Compositae
Keyword: Phytochemistry,
Pharmacology,
Oncology,
Essential oils,
Chemical composition,
Antineoplastic activity,
Cervical cancer,
Cytotoxicity,
Tagetes ostenii,
Compositae
Full text: Texto completo (Ver HTML) Texto completo (Ver PDF)