| Journal: | Acta cirurgica brasileira |
| Database: | PERIÓDICA |
| System number: | 000271972 |
| ISSN: | 0102-8650 |
| Authors: | Souza, Mardem Machado de1 Aguilar-Nascimento, Jose Eduardo de2 Dock-Nascimento, Diana Borges3 |
| Institutions: | 1Universidade de Cuiaba, Faculdade de Medicina, Cuiaba, Mato Grosso. Brasil 2Universidade Federal de Mato Grosso, Departamento de Cirurgia, Cuiaba, Mato Grosso. Brasil 3Universidade Federal de Mato Grosso, Escola de Nutricao, Cuiaba, Mato Grosso. Brasil |
| Year: | 2007 |
| Volumen: | 22 |
| Pages: | 40-45 |
| Country: | Brasil |
| Language: | Inglés |
| Document type: | Artículo |
| Approach: | Experimental |
| English abstract | PURPOSE: The aim of this study was to investigate the effect of enemas containing probiotics and budesonide on the systemic inflammatory response in experimental colitis. METHODS: Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1 - saline solution, group 2 - budesonide (0.75 mg/kg/day), group 3 - probiotics (1mg/day), group 4 - probiotics plus budesonide, and group 5 - control, with not-treated rats. The following variables were studied: body weight, serum levels of albumin, C-reactive protein and interleucine-6 (IL-6). RESULTS: All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was a significant decrease in the serum albumin between the normal pre-induction level (3.45 + 0.49mg/dL) and the 1st day after colitis induction (1.61+051mg/dL, p< 0.001) in all treated groups when compared to the control group. C- reactive protein increased after induction and diminished on the 7th day in all groups. In the control group there was an increase in the IL-6 after colitis induction. None of the treated groups significantly differed from IL-6 pre-colitis status (p>0.05). Only probiotic rats presented a significant decrease of IL-6 than controls (0,30±0,08 mg/dL vs. 0,19±0,03 mg/dL; p<0.01). CONCLUSION: Probiotic associated with budesonida Probiotics are effective to diminished inflammatory status mediated by IL-6 in experimental colitis |
| Portuguese abstract | OBJETIVO: Investigar o efeito da administração retal de probióticos e budesonida na resposta inflamatória de ratos com colite experimental. MÉTODOS: Cinqüenta ratos Wistar com colite experimental induzida pelo acido acético à 10% foram randomizados em 5 grupos (n=10 por grupo) para diferentes tratamentos: grupo 1 - solução fisiológica; grupo 2 budesonida (0,75mg/kg/dia); grupo 3 - probióticos (1 g/dia); grupo 4 - probióticos associados a budesonida; e finalmente grupo 5 - controle, composto por ratos sem tratamento. As seguintes variáveis foram estudadas: peso corporal, dosagens séricas de albumina, proteína C reativa (PCR) e interleucina-6 (IL-6). RESULTADOS: CONCLUSÃO: Probióticos são efetivos na diminuição do estado inflamatório mediado pela IL-6 na colite experimental |
| Disciplines: | Medicina |
| Keyword: | Gastroenterología, Medicina experimental, Terapéutica y rehabilitación, Probióticos, Mucosas, Colon, Interleucina-6, Proteína C reactiva, Budesonida, Colitis ulcerosa, Respuesta inflamatoria |
| Keyword: | Medicine, Experimental medicine, Gastroenterology, Therapeutics and rehabilitation, Probiotics, Interleukin-6, Mucosae, Colon, C reactive protein, Ulcerative colitis, Budesonide, Inflammatory response |
| Full text: | Texto completo (Ver HTML) |
