Effects of budesonide and probiotics enemas on the systemic inflammatory response of rats with experimental colitis



Título del documento: Effects of budesonide and probiotics enemas on the systemic inflammatory response of rats with experimental colitis
Revista: Acta cirurgica brasileira
Base de datos: PERIÓDICA
Número de sistema: 000271972
ISSN: 0102-8650
Autors: 1
2
3
Institucions: 1Universidade de Cuiaba, Faculdade de Medicina, Cuiaba, Mato Grosso. Brasil
2Universidade Federal de Mato Grosso, Departamento de Cirurgia, Cuiaba, Mato Grosso. Brasil
3Universidade Federal de Mato Grosso, Escola de Nutricao, Cuiaba, Mato Grosso. Brasil
Any:
Volum: 22
Paginació: 40-45
País: Brasil
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental
Resumen en inglés PURPOSE: The aim of this study was to investigate the effect of enemas containing probiotics and budesonide on the systemic inflammatory response in experimental colitis. METHODS: Fifty male Wistar rats with experimental colitis induced by 10% acetic acid enema were randomized to five groups (10 rats each) according to the treatment: group 1 - saline solution, group 2 - budesonide (0.75 mg/kg/day), group 3 - probiotics (1mg/day), group 4 - probiotics plus budesonide, and group 5 - control, with not-treated rats. The following variables were studied: body weight, serum levels of albumin, C-reactive protein and interleucine-6 (IL-6). RESULTS: All animals lost weight between the beginning and the end of the experiment (280+ 16 mg versus 249+21 mg, p< 0.001). There was a significant decrease in the serum albumin between the normal pre-induction level (3.45 + 0.49mg/dL) and the 1st day after colitis induction (1.61+051mg/dL, p< 0.001) in all treated groups when compared to the control group. C- reactive protein increased after induction and diminished on the 7th day in all groups. In the control group there was an increase in the IL-6 after colitis induction. None of the treated groups significantly differed from IL-6 pre-colitis status (p>0.05). Only probiotic rats presented a significant decrease of IL-6 than controls (0,30±0,08 mg/dL vs. 0,19±0,03 mg/dL; p<0.01). CONCLUSION: Probiotic associated with budesonida Probiotics are effective to diminished inflammatory status mediated by IL-6 in experimental colitis
Resumen en portugués OBJETIVO: Investigar o efeito da administração retal de probióticos e budesonida na resposta inflamatória de ratos com colite experimental. MÉTODOS: Cinqüenta ratos Wistar com colite experimental induzida pelo acido acético à 10% foram randomizados em 5 grupos (n=10 por grupo) para diferentes tratamentos: grupo 1 - solução fisiológica; grupo 2 budesonida (0,75mg/kg/dia); grupo 3 - probióticos (1 g/dia); grupo 4 - probióticos associados a budesonida; e finalmente grupo 5 - controle, composto por ratos sem tratamento. As seguintes variáveis foram estudadas: peso corporal, dosagens séricas de albumina, proteína C reativa (PCR) e interleucina-6 (IL-6). RESULTADOS: CONCLUSÃO: Probióticos são efetivos na diminuição do estado inflamatório mediado pela IL-6 na colite experimental
Disciplines Medicina
Paraules clau: Gastroenterología,
Medicina experimental,
Terapéutica y rehabilitación,
Probióticos,
Mucosas,
Colon,
Interleucina-6,
Proteína C reactiva,
Budesonida,
Colitis ulcerosa,
Respuesta inflamatoria
Keyword: Medicine,
Experimental medicine,
Gastroenterology,
Therapeutics and rehabilitation,
Probiotics,
Interleukin-6,
Mucosae,
Colon,
C reactive protein,
Ulcerative colitis,
Budesonide,
Inflammatory response
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