| Revue: | Veterinaria México OA |
| Base de datos: | PERIÓDICA |
| Número de sistema: | 000439071 |
| ISSN: | 2007-5472 |
| Autores: | Martínez Ibarra, José Luis1 Espinoza Mendoza, Eugenia Adriana1 Rangel Santos, Raymundo2 Ambriz Garcia, Demetrio Alonso1 Navarro Maldonado, María del Carmen1 |
| Instituciones: | 1Universidad Autónoma Metropolitana, División de Ciencias Biológicas y de la Salud, Iztapalapa, Ciudad de México. México 2Universidad Autónoma Chapingo, Departamento de Zootecnia, Chapingo, Estado de México. México |
| Año: | 2018 |
| Periodo: | Oct-Dic |
| Volumen: | 5 |
| Número: | 4 |
| País: | México |
| Idioma: | Inglés |
| Tipo de documento: | Artículo |
| Enfoque: | Experimental, analítico |
| Resumen en inglés | The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos |
| Disciplinas: | Medicina veterinaria y zootecnia, Biología |
| Palabras clave: | Ovinos y caprinos, Fisiología animal, Reproducción y desarrollo, Resveratrol, Ovis aries, Ovejas, Ovocitos, Maduración in vitro, Embriones, Clonación |
| Keyword: | Sheep and goats, Animal physiology, Reproduction and development, Resveratrol, Ovis aries, Ewes, Oocytes, In vitro maturation, Embryos, Cloning |
| Texte intégral: | Texto completo (Ver HTML) Texto completo (Ver PDF) |
