Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos



Título del documento: Effect of resveratrol on the in vitro maturation of ovine (Ovis aries) oocytes and the subsequent development of handmade cloned embryos
Revista: Veterinaria México OA
Base de datos: PERIÓDICA
Número de sistema: 000439071
ISSN: 2007-5472
Autores: 1
1
2
1
1
Instituciones: 1Universidad Autónoma Metropolitana, División de Ciencias Biológicas y de la Salud, Iztapalapa, Ciudad de México. México
2Universidad Autónoma Chapingo, Departamento de Zootecnia, Chapingo, Estado de México. México
Año:
Periodo: Oct-Dic
Volumen: 5
Número: 4
País: México
Idioma: Inglés
Tipo de documento: Artículo
Enfoque: Experimental, analítico
Resumen en inglés The effect of resveratrol on the in vitro maturation (IVM) of ovine (Ovis aries) oocytes and the development of handmade cloned embryos was evaluated. The nuclear maturation and reactive oxygen species (ROS) levels in the oocytes, as well as the early development and morphological cloned embryo quality, were evaluated under different resveratrol concentrations (0, 0.5, 2 and 5 µM). After IVM, no significant difference was observed in the maturation rate of oocytes treated with 0.5 µM (81.3 %) and 2 µM (72 %) resveratrol compared to that of the control group (0 µM) (74.2 %), but the rate significantly decreased at 5 µM (56 %) (p < 0.05). When the oocyte ROS levels were determined, no significant differences among the groups were observed (p > 0.05). For cloned embryo development, the embryos obtained from the oocytes treated with 0.5 µM resveratrol showed higher (p < 0.05) compacted morula rates (10.7 %) compared to the embryos obtained from the oocytes treated with 0, 2 and 5 µM (6.2, 0 and 0 %, respectively). Regarding embryo morphological quality, the embryos from the oocytes treated with 0.5 µM resveratrol showed a lower rate of poor quality morulae (4.7 %) in comparison to those treated with 0, 2 and 5 µM (23.8, 23.3 and 33.3 %, respectively) (p < 0.05). In conclusion, resveratrol showed no significant improvement on the IVM or ROS levels in domestic ovine oocytes. However, treatment with 0.5 µM resveratrol during IVM improved embryo quality and promoted morulae compaction of Ovis aries handmade cloned embryos
Disciplinas: Medicina veterinaria y zootecnia,
Biología
Palabras clave: Ovinos y caprinos,
Fisiología animal,
Reproducción y desarrollo,
Resveratrol,
Ovis aries,
Ovejas,
Ovocitos,
Maduración in vitro,
Embriones,
Clonación
Keyword: Sheep and goats,
Animal physiology,
Reproduction and development,
Resveratrol,
Ovis aries,
Ewes,
Oocytes,
In vitro maturation,
Embryos,
Cloning
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